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. 2008 Dec;17(6):1109–1121. doi: 10.1089/scd.2008.0068

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Copyright 2008, Mary Ann Liebert, Inc.

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FIG. 1. — Derivation and characterization of human adult bone-marrow-derived mesenchymal stem cells (MSCs). (A) Morphology of the MSCs derived from human adult BM samples. (B) Flow cytometric analysis for the expression of characteristic MSC antigens including CD29, CD44, CD73, CD90, CD105, CD166, CD34, CD45, CD14, and CD11. Red line represents the isotype control and green line represents the staining against each specified antibodies. (C) Differentiation of MSCs into three mesodermal lineages: adipocytes (oil red O staining), chondrocytes (collagen II antibody staining), and osteoblasts (von Kossa staining for calcium deposits). Scale bars: (A) 100 μm; (C) adipocyte 10 μm, osteoblast 8 μm, and chondrocyte 50 μm.