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. 2008 Dec;17(6):1109–1121. doi: 10.1089/scd.2008.0068

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Copyright 2008, Mary Ann Liebert, Inc.

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FIG. 3. — Characterization of mesenchymal stem cell (MSC)-derived neural stem cells (NSCs). (A) Immunocytochemical analysis for the expression of nestin and musashi-1 on MSC-derived NSCs. FITC-labeled secondary antibody was used for both nestin and musashi-1. DAPI was used for nuclear staining. (B) Flow cytometric analysis for the expression characteristic MSC antigens on MSC-derived NSCs. Antigens include CD29, CD44, CD73, CD90, CD105, and CD166. Red line represents the isotype control and green line represents the staining against each specified antibodies. (C) Differentiation of MSC-derived NSCs into three neural lineages including neurons (β-tubulin III; FITC-labeled secondary antibody), astrocytes (glial fibrillary acid protein; FITC-labeled secondary antibody), and oligodendrocytes (galactosylceramidase; Texas Red-labeled secondary antibody). DAPI was used for nuclear staining. Scale bars: (A) 50 μm; and (C) 50 μm.