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. 2008 Dec;17(6):1109–1121. doi: 10.1089/scd.2008.0068

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Copyright 2008, Mary Ann Liebert, Inc.

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FIG. 5. — Characterization of mesenchymal stem cells (MSCs) generated from MSC-derived neural stem cells (NSCs). (A) Morphology of adherent cells generated from MSC-derived NSCs in MSC culture condition. (B) Flow cytometric analysis for the expression of characteristic MSC antigens on the adherent cells generated from MSC-derived NSCs in MSC culture condition. Characteristic MSC antigens include CD29, CD44, CD73, CD90, CD105, and CD166. Red line represents the isotype control and green line represents the staining against each specified antibodies. (C) Differentiation of adherent cells generated from MSC-derived NSCs into three mesodermal lineages: adipocytes (oil red O staining), chondrocytes (collagen II antibody staining), and osteoblasts (von Kossa staining for calcium deposits). Scale bars: (A) 75 μm; (C) adipocyte 2.5 μm, osteoblast 15 μm, and chondrocyte 50 μm.