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Differentiated mesenchymal stem cells (MSCs) continue to express GFP. MSCs were differentiated to adipocytes and stained for intracellular lipid-rich vacuoles by Oil Red O (inset). Phase microscopy (A), fluorescent microscopy in the 500–530 nm emission wavelengths (B), and an overlay of both (C) is used to show two MSCs differentiated to adipocytes: arrowhead 1 shows a cell that maintained GFP expression and arrowhead 2 shows a cell that maintained GFP nonexpression.
