FIG. 7.

Flow cytometric assessment of the role of histone acetylation in GFP expression. Mesenchymal stem cells were sorted, expanded, and grown in the presence of trichostatin A (TSA), a histone deacetylase inhibitor, at a concentration of 0.01, 0.1, 0.5, and 1.0 μM for 12, 24, or 48 h. Only the higher concentrations at the later time points lead to increases in histone acetylation and are shown here. Although increases in histone acetylation, H3 > H4, are observed, there are minimal increases in GFP expression. H3: histone H3; H4: histone H4; HAc: histone acetylation.