Table 1.
Characteristics of the 21 microsatellite loci isolated from Atrina pectinata.
| Locus | Repeat Motif | Primer Sequence (5′→3′) | Ta (°C) | Genebank Accession No. |
|---|---|---|---|---|
| KAp2 | (GT)12 | F: CAATGTTGATGATGGATGTTA ned R: GCTTCATGTGGGTTTGG |
60 | EU026353 |
| KAp6 | (GT)12 | F: CAGCTACCAAAACCATAAATC hex R: CCAGTCAGCTTGAGTTACAGA |
60 | EU026354 |
| KAp9 | (GT)9 | F: AATGGTACAGTTGTACAGCAC ned R: CAAGCATATTTGTCATTTGAT |
55 | EU026355 |
| KAp11 | (CA)12 | F: GCTGTTGGAAATACGGACTAC 6-fam R: CCACCAAAATTCGGTAAAA |
55 | EU026356 |
| KAp12 | (GT)13 | F: CGATCCTATCCAAGGGTTAT 6-fam R: CTCTATCCGTTCTCCATTTCT |
55 | EU026357 |
| KAp17 | (CA)11 | F: GCAAGGCAAAATGTATTACC 6-fam R: CGAGTACTTGCCGTAGTGAC |
55 | EU026358 |
| KAp18 | (CA)12 | F: TTGGAAATACGGACTACTCA hex R: CCTTGACGTGACCACTTA |
57 | EU026359 |
| KAp19 | (GT)15 | F: CAAAATCCAGGAGTATCTCA hex R: TTCCCTAGAGTGCATAAACT |
55 | EU026360 |
| KAp20 | (GT)11 | F: ACCGTAGTGACACTGAAGGA hex R: GAGGCAAGGCAAAATGTAT |
55 | EU026361 |
| KAp21 | (GT)15 | F: GGAATCATTCTCGCAATA 6-fam R: GAAGCACGTATCATCACTAA |
55 | EU026362 |
| KAp23 | (CA)11 | F: ATCAAGTCATTGCCACAC ned R: AGAAGCACTTGCCGTAG |
57 | EU026363 |
| KAp24 | (GT)13 | F: CCGTGCTGTGGTAATGTA hex R: TTGGCATAAATAGAAAGGTT |
52 | EU026364 |
| KAp25 | (CA)22 | F: CGCGTTCGACTCTTGA ned R: CAAAATTTGGCCTATGCT |
52 | EU026365 |
| KAp26 | (GT)10 | F: GGCGTGTCTATACTTGAACT 6-fam R: TGTCACTTGCGTCACTTTA |
55 | EU026366 |
| KAp30 | (GT)11 | F: TTGAACAAAGACTTGTCA ned R: TCACGTTGAGACTTCATA |
52 | EU026367 |
| KAp32 | (CA)13 | F: TCATCATGTGGCTGTATA ned R: CTGCAGTTGCTTGAAG |
55 | EU026368 |
| KAp33 | (GT)14AA(GT)4 | F: CCACCTGACTGTCTCTGA hex R: ACAGAATCTCGCCTAAAG |
52 | EU026369 |
| KAp38 | (GT)CT(GT)13 | F: CGCCTACATTTAGTCAGT ned R: CTTGGCTTGTACCATATC |
50 | EU026370 |
| KAp39 | (GT)21T(GT) | F: CCGACGTATTATTAGTGC 6-fam R: GCGTAACCCATGTATTAA |
50 | EU026371 |
| KAp40 | (GT)10TT(GT)4 | F: CCATTCACCAAGAGGTTG ned R: CCGTGCGTTGTCGTAC |
50 | EU026372 |
| KAp42 | (GA)3(GT)8 | F: GATAGGCTTCCGTTGTTCTA hex R: AGCCTATGTTCCACGAGAC |
55 | EU026373 |
| KAp43 | (TG)8 | F: AGCTGTTTCACTCTCATTT 6-fam R: AGAATTTTAACCACAACCTT |
52 | EU026374 |
Ta is the optimal annealing temperature.