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emb-1 mutants arrest at metaphase of meiosis I. (A) emb-1(hc62ts); tubulin::gfp animal at 16°. Bar, ∼20 μm (A and B). (B) emb-1(hc62ts); tubulin::gfp animal at 24°. (C) Embryo from mother injected with K10D2.4 dsRNA and stained with anti–α-tubulin antibodies. Bar, ∼10 μm (C and D). (D) Same embryo stained with TOTO-3 to visualize the oocyte and sperm chromosomes. Oocyte chromosomes in metaphase I are marked with a white arrow.
