Table 3 . The emb-1 mutant is suppressed by SAC and fzy-1 mutations.
| Genotype | N | Phenotype @ 24° |
|---|---|---|
| unc-42; emb-1(hc62ts) lon-1 | 400 | One-cell embryos; 0% hatch |
| mdf-1(av19) unc-42; emb-1(hc62ts) lon-1 | 1012 | Unh MC embryos; 0% hatch |
| mdf-1(av19) unc-42; emb-1(hc62ts) | 447 | Unh MC embryos; 3% hatch |
| unc-17; emb-1(hc62ts) lon-1 | 589 | One-cell embryos; 0% hatch |
| mdf-2(av16) unc-17; emb-1(hc62ts) lon-1 | 405 | Unh MC embryos; 2% hatch |
| mdf-2(av16) unc-17; emb-1(hc62ts) | 387 | Unh MC embryos; 1 hatched |
| unc-13; emb-1(hc62ts) lon-1 | 200 | One-cell embryos; 0% hatch |
| mdf-3(av31) unc-13; emb-1(hc62ts) lon-1 | 1567 | Unh MC embryos; 1 hatched |
| mdf-3(av31) unc-13; emb-1(hc62ts) | 236 | Unh MC embryos; 2% hatch |
| unc-4; emb-1(hc62ts) lon-1 | 712 | One-cell embryos; 0% hatch |
| fzy-1(av15gf) unc-4; emb-1(hc62ts) lon-1 | 1395 | Unh MC embryos; 1% hatch |
| fzy-1(av15gf) unc-4; emb-1(hc62ts) | 504 | Unh MC embryos; 1% hatch |
Double mutants of emb-1 with previously identified SAC mutants or a gain-of-function fzy-1 allele. Mothers were identified on the basis of their linked morphological markers. The generation of these double mutants was carried out at 15°. L4 larvae of the appropriate genotype were shifted to 24° to assay for suppression of the emb-1 one-cell phenotype. The phenotype indicated is for the progeny of the doubly mutant mother. N = number of embryos counted. Unh MC, unhatched multicellular embryos, which indicates suppression at the cellular level.