Table 4 . Number of M-phase nuclei in germline proliferative zone.
| Genotype | Growth conditions | N | pHisH3(S10) nuclei/arm |
|---|---|---|---|
| Wild type (N2) | 9 hr at 25° | 60 | 4.4 ± 3.5 |
| emb-1 (hc57ts) /+ | 9hr at 25° | 29 | 3.9 ± 1.9 |
| emb-1 (hc62ts) | Constant 16° | 52 | 6.4 ± 3.6 |
| emb-1 (hc62ts) | 6 hr at 25° | 36 | 6.2 ± 2.8 |
| emb-1 (hc57ts) | 9 hr at 25° | 68 | 8.7 ± 3.5 |
| emb-1 (hc57ts)/ok2759 | 9 hr at 25° | 32 | 8.0 ± 2.4 |
Strains and crosses were maintained at 16° before young adult hermaphrodites were shifted to 25° for the indicated duration. Animals were dissected to isolate gonadal arms, processed for immunofluorescence, and pHisH3(S10) positive nuclei were scored under epifluorescence.