Intrinsic and GTPγS-induced facilitation of
Cav2.2b(G177E) channels. (A) Prepulse
facilitation of the rate of activation without GTPγS, measured as in
Fig. 2A. (B) Prepulse
facilitation of the voltage dependence of activation without GTPγS.
Tail currents were recorded following test 1 (▴) and test 2
(▵) at the indicated membrane potentials as described in
A. Mean ± SEM of tail currents are plotted vs.
test potential. (C) Prepulse facilitation of the rate of
activation with 0.2 mM GTPγS in the intracellular solution, measured
as in A. (D) Prepulse facilitation of the
voltage dependence of activation with 0.2 mM GTPγS, measured as in
B. (Scale bars: A, 0.2 nA, 4 ms;
B, 0.16 nA, 4 ms.)