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. 2011 Oct;189(2):423–439. doi: 10.1534/genetics.111.131326

Table A1. Frequency of tetrads with at least two detectable recombination events.

MSH5 genotype SPO11 genotype No. of NPD (%) No. of TNCO(%)
Wild type Wild type 4/462 (0.9) 24/462 (5.2)
spo11-HA 9/745 (1.2), P = 0.78 44/745 (5.9), P = 0.70
spo11-yf 16/743 (2.2), P = 0.11 44/743 (5.9), P = 0.70
msh5Δ Wild type 6/766 (0.8), P = 1.00 31/766 (4.0), P = 0.39

Gfp*+ tetrads were enriched by FACS from one culture for wild type, spo11-HA, and spo11-yf and from two independent cultures for msh5Δ (≥302 tetrads analyzed per culture). Statistical significance was evaluated by a Fisher’s exact test (two-tailed P-value) that compared each strain to wild type. Note that the observed NPDs may underrepresent total NPDs because a small fraction of tetrads that appear to display MI nondisjunction (Figure 5G) could have arisen from a four-strand double crossover instead. Such NPD tetrads are expected to be exceedingly rare, however, because they contain a Gfp*+ spore that is also Rfp+ Cfp+, which is an uncommon configuration (≥14-fold less common than the Rfp Cfpconfiguration; see Table 2A). All such tetrads were thus scored as “MI nondisjunction.”