Table A2. Estimates of values of k from tetrad data.
Strain | k1 | k2 | COobs | Total | %COobs | %COcorr |
---|---|---|---|---|---|---|
Wild type | 0.543 | 0.591 | 623 | 1013 | 61.5 | 59.1–59.2 |
spo11-HA | 0.555 | 0.617 | 603 | 1026 | 58.8 | 56.8–57.1 |
spo11-yf | 0.552 | 0.610 | 690 | 1034 | 66.7 | 67.7–68.2 |
msh5Δ | 0.507 | NAa | 443 | 1063 | 41.7 | 40.0 |
Values of k were calculated for scenario 1 (k1) and 2 (k2) (Figure A2B) using the formula in Figure A2C. The corrected fraction of GFP* conversions that are crossover associated (%COcorr) was calculated using Equation 4.
Because msh5Δ strains do not show crossover interference, k2 (calculated assuming complete interference) is not applicable.