Figure 5 .

Mtap1a is a candidate gene for Mdwh. (A) Genetic structure of Mtap1a showing the position of a polymorphic repeat sequence (vertical yellow bar) in the large exon 5. (B) Primers (highlighted in blue) were designed to amplify the GCTCCA repeat sequence (highlighted in yellow; from C57BL/6J DNA), which encodes an alanine–proline repeat. (C) Sequencing of the 268-bp PCR amplification products from CAST/EiJ (lane 1) and C3H/HeJ (lane 2) showed that these strains have 15 repeats of the 6-bp sequence, whereas the 208-bp PCR product from the DW strain (lane 3) showed only 5 repeats.