Figure 2 .
Contrasting site-specific nucleotide frequencies between highly expressed genes (500 genes with the highest CAI values) and lowly expressed genes (500 genes with the lowest CAI values). The increase in A usage toward the initiation AUG is visually more dramatic in highly expressed genes than lowly expressed genes. The nucleotide frequency distribution between HEG and LEG are highly significantly different (χ2 = 592.74, DF = 3, P < 0.0001).