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. 2011 Oct;189(2):675–679. doi: 10.1534/genetics.111.130971

Figure 2.

Figure 2

P. xylostella ABCC2 schematic. A P. xylostella BAC library was screened with partial gene sequence from ABCC2, which was identified from an EST library, and two clones were sequenced (25L19 and 11G15). P. xylostella ABCC2 genomic sequence was predicted on the basis of similarity with the H. virescens homolog and then confirmed through amplification from midgut cDNA, using backcross progeny that survived a Cry1Ac bioassay (Bt-resistant) or from the control strain Geneva88 (Bt-susceptible). (A) Partial amino acid alignment of ABCC2 of Bt-susceptible (JN030490) and Bt-resistant (JN030491) P. xylostella. A 30-bp, 10-amino-acid deletion is predicted within transmembrane (TM) domain 12. (B) Schematic of ABCC2 displaying 12 transmembrane domains predicted using Phobius (http://phobius.sbc.su.se/) and two nucleotide-binding domains (NBD). The 10-amino-acid deletion in Bt-resistant individuals is predicted to remove TM12, leaving the carboxyl terminus in a noncytoplasmic region (dashed line).