Table 1.
Proteins differentially expressed in the second trimester in the pH range 4.5 - 5.5
| Spot no. (as on Figure 1) | Accession number (Swiss-Prot) | Abbreviated name | Protein name | Average fold up-regulation (DS/Ctl) | Theoretical mW (Da) | Theoretical pI | Scores for individual spot identifications | Number of sequenced peptides | Peptide positions within the protein | Peptides sequenced (overlap between spots identified per protein) | Coverage (%) (Total number of peptides assigned to protein) | Function |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | P00450 | Ceruloplasmin (Seven spots identified for this protein) |
CERU_HUMAN | 3.69 | 122205 | 5.44 | 173, 437, 431, 460, 331, 107, 110 | 8, 17, 16, 15, 12, 7, 7 | 42 - 63 43 - 63 69 - 82 187 - 202 248 - 259 426 - 437 468 - 482 484 - 501 523 - 538 537 - 548 537 - 559 547 - 559 598 - 610 598 - 620 720 - 733 785 - 794 798 - 820 887 - 902 944 - 958 |
K.KLISVDTEHSNIYLQNGPDR.I K.LISVDTEHSNIYLQNGPDR.I K.ALYLQYTDETFR.T K.DIASGLIGPLIICK.K K.DNEDFQESNR.M R.EYTDASFTNR.K K.GAYPLSIEPIGVR.F K.NNEGTYYSPNYNPQSR.S K.EVGPTNADPVCLAK.M K.MYYSAVDPTK.D K.MYYSAVDPTKDIFTGLIGPMK.I K.DIFTGLIGPMK.I R.MFTTAPDQVDK.E R.MFTTAPDQVDKEDEDFQESNK.M R.QSEDSTFYLGER.T R.QYTDSTFR.V R.KAEEEHLGILGPQLHADVGDK.V K.DLYSGLIGPLIVCR.R K.VNKDDEEFIESNK.M |
20.6 (19) | Copper-binding glycoprotein, ferroxidase activity, iron transport |
| 2 | Q14624 | inter-alpha trypsin inhibitor heavy chain H4 | ITIH4_HUMAN | 2.11 | 103357 | 6.51 | 74 | 10 | 98 - 112 152 - 163 214 - 225 299 - 308 428 - 439 500 - 513 633 - 645 657 - 670 815 - 827 831 - 843 |
K.AEAQAQYSAAVAK.G R.RLGVYELLLK.V R.FKPTLSQQQK.S K.ILDDLSPR.D K.LALDNGGLAR.R R.GPDVLTATVSGK.L K.IPKPEASFSPR.R R.MNFRPGVLSSR.L K.ETLFSVMPGLK.M K.TGLLLLSDPDK.V |
11.5 (10) | Serine-type endopeptidase inhibitor, acute-phase response, hyaluronan metabolic process |
| 3 | P0C0L4 | complement C4-A precursor (Two spots identified for this protein) | CO4A_HUMAN | 2.61 | 192771 | 6.66 | 192, 93 | 8, 4 | 756 - 776 916 - 930 979 - 1006 1042 - 1052 1084 - 1100 1291 - 1301 1340 - 1350 1352 - 1366 |
R.ALEILQEEDLIDEDDIPVR.S R.GSFEFPVGDAVSK.V R.VTASDPLDTLGSEGALSPGGVASLLR.L K.DHAVDLIQK.G K.VLSLAQEQVGGSPEK.L R.QGSFQGGFR.S K.SHALQLNNR.Q R.GLEEELQFSLGSK.I |
6.5 (8) | Activation of the classical pathway of the complement system |
| 4 | P01031 | complement C5 precursor | CO5_HUMAN | 1.59 | 188305 | 6.11 | 154 | 6 | 78 - 91 308 - 322 353 - 373 436 - 450 495 - 507 622 - 630 |
K.FQNSAILTIQPK.Q K.ELSYYSLEDLNNK.Y K.LNLVATPLFLKPGIPYPIK.V K.TDAPDLPEENQAR.E K.ITHYNYLILSK.G R.VFQFLEK.S |
4.5 (6) | Complement cascade through to formation of membrane attack complex (MAC), inflammatory response |
| 5 | P02748 | complement component C9 precursor | CO9_HUMAN | 2.38 | 63173 | 5.43 | 196 | 7 | 65 - 77 144 - 155 145 - 155 231 - 243 472 - 484 496 - 509 533 - 544 |
R.SIEVFGQFNGK.R R.DRVVEESELAR.T R.VVEESELAR.T K.TSNFNAAISLK.F K.LSPIYNLVPVK.M R.AIEDYINEFSVR.K K.FEGIACEISK.Q |
11.8 (7) | Pore-forming subunit of the MAC that plays a key role in the innate and adaptive immune response |
| 6 | P01042 | kininogen-1 (Two spots identified for this protein) | KNG1_HUMAN | 1.72 | 71957 | 6.34 | 37, 187 | 6, 9 | 43 - 59 64 - 76 101 - 114 187 - 197 208 - 220 240 - 255 254 - 263 316 - 325 380 - 390 |
K.YNSQNQSNNQFVLYR.I K.TVGSDTFYSFK.Y K.AATGECTATVGK.R R.QVVAGLNFR.I K.ENFLFLTPDCK.S R.IASFSQNCDIYPGK.D K.DFVQPPTK.I K.YFIDFVAR.E K.RPPGFSPFR.S |
15.1 (9) | Role in blood coagulation, inhibitor of thiol proteases, inflammatory response, diuresis and natriuresis, smooth muscle contraction, negative regulation of cell adhesion |
| 7 | P09871 | complement C1s subcomponent precursor (Three spots identified for this protein) | C1S_HUMAN | 4.45 | 76684 | 4.86 | 190, 66 | 8, 5, 6 | 86 - 106 264 - 281 314 - 332 369 - 384 481 - 497 515 - 524 523 - 535 629 - 645 677 - 688 |
R.SSNNPHSPIVEEFQVPYNK.L K.SNALDIIFQTDLTGQK.K R.DVVQITCLDGFEVVEGR.V K.VEDPESTLFGSVIR.Y R.EPTMYVGSTSVQTSR.L K.LLEVPEGR.T R.TNFDNDIALVR.L K.GDSGGAFAVQDPNDK.T K.TMQENSTPRED.- |
18.3 (9) | Serine-type endopeptidase involved in activation of classical pathway of the complement system |
The table shows those proteins that are differentially expressed between DS and Ctl pregnancies in the second trimester in the pH range 4.5 - 5.5. All results displayed here are from ESI Q-TOF MS/MS analysis. Identified proteins are given with UniProt accession number, average fold up-regulation in DS samples compared to Ctl samples (from DeCyder analysis; comparable to Progenesis), theoretical molecular weight and pI (calculated using the Compute pI/mW function available on the ExPASy website, full amino acid sequence used including signal peptides), search score and number of peptides used for identification. The sequences of the peptides used for the identification of the protein spots (M indicates methionine oxidation) and their position within the protein sequence are shown; also the function of the protein. Coverage indicates the percentage of the residues in each protein sequence that was identified. Score is -10*Log(P), where P is the probability that the observed match is a random event. Scores > 46 indicate identity or extensive homology at the p < 0.05 level.