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. 2011 Oct 10;6(10):e25427. doi: 10.1371/journal.pone.0025427

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Batut et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, C) Dorsal flat-mounted 14 ss embryos (anterior to top) injected with the indicated Mo were analyzed by whole-mount in situ hybridization for (A) mlc2f and (C) smyhc1 or (B, D) immunohistochemistry for (B) fast fibers, (D) slow fibers. In (C) a magnification of the area indicated by the black line is shown to the right of each panel. Arrow or white arrowhead, lateral expression; red arrowhead, medial expression; red bar, length of one somite. (B, D) Z-stack sections of the most anterior somites are shown.