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. 2011 Oct 10;6(10):e25925. doi: 10.1371/journal.pone.0025925

Figure 7. JA-Ile, but not JA complementations, restored local TPI activity but only partially nicotine and DTG production in 35S-jmt plants.

Figure 7

Mean (± SD, n = 5) (A) trypsin proteinase inhibitor (TPI) activity, (B) nicotine- and (C) diterpene glycoside (DTGs) accumulation in local and systemic tissues of induced rosette-stage wild-type (WT, black bars) plants and plants ectopically expressing AtJMT (35S-jmt-1, white bars; 35S-jmt-2, grey bars) harvested 3 days after one fully expanded leaf per plant was wounded by a fabric pattern wheel and treated with distilled water (W+W), JA (0.1 µmoles, W+JA) or JA-Ile (0.1 µmoles, W+JA-Ile). The orthostichous leaf above the treated leaf (Local) was analyzed for the systemic response in elicited plants (Systemic). Dashed lines indicate W+W level of 35S-jmt-1 (grey) and 35S-jmt-2 (black). Bars sharing the same letters are not significantly different (unpaired t-test).