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. 2011 Oct 10;6(10):e26116. doi: 10.1371/journal.pone.0026116

Figure 2. Young Risp +/P224S mice have decreased RISP protein and complex III enzymatic activity.

Figure 2

(A) A representative western blot showing RISP protein levels in mitochondria isolated from liver of 3-month-old female adults. A wild-type/heterozygous littermate pair is shown (‘m’ denotes the knock-in mutant allele P224S). (B) Densitometry was used to quantify RISP relative to porin. All measurements were made in wild-type/heterozygote littermate pairs, and results are expressed as the effect of Risp heterozygosity relative to wild-type within each pair. (C) Enzymatic activity of complexes of the mitochondrial ETC (complex I, II, III or IV) was determined in disrupted mitochondria, using appropriate substrates and inhibitors to isolate the activity of each complex. All measurements were made in wild-type/heterozygote littermate pairs, and results are expressed as the effect of Risp heterozygosity relative to wild-type within each pair. All results are for male mice, except where indicated (as F, for female). Actual densitometry values for (A) and enzymatic activities for all samples (C) are shown in Table S2. Sample sizes (i.e., number of wild-type/heterozygous littermate pairs) are shown superimposed on each bar. ‘**’ denotes p = 0.001 to 0.01 and ‘***’ denotes p<0.001 vs. 1.0.