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. 2011 Nov 1;204(Suppl 3):S840–S849. doi: 10.1093/infdis/jir306

Figure 3.

Figure 3.

Expression of Ebola virus glycoproteins (EBOV-GPs) induces detachment of human and bat cells. A, 293T cells were transfected with pCAGGS plasmids encoding EBOV-GP-V5 or empty pCAGGS plasmid (Mock), and the number of cells in the culture supernatant was counted at 48 hours after transfection via fluorescence-activated cell sorting. The mean of 12 independent experiments is shown. Error bars indicate standard error of the mean (SEM). B, EpoNi/22.1 cells were transfected with pCAGGS plasmids encoding EBOV-GP-V5, and the number of cells in the supernatant was counted as described in A. The mean of 11 independent experiments is shown. Error bars indicate SEM. C, 293T cells were transfected with pCAGGS or pcDNA plasmids encoding EBOV-GPs with C-terminal V5 tag and treated as in Figure 1. D, 293T cells were transfected with pcDNA plasmids encoding EBOV-GP-V5, and the number of cells in the supernatant was counted as in A. The mean of 11 independent experiments is shown. Error bars indicate SEM. Asterisks indicate statistically significant differences (P < .05) relative to mock as determined by means of 2-tailed Student t test. CIEBOV, Côte d’Ivoire ebolavirus; REBOV, Reston ebolavirus; SEBOV, Sudan ebolavirus; ZEBOV, Zaire ebolavirus.