Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Intracellular localization of the L domain motif mutants. We transfected 293 cells with plasmids expressing cMyc-tagged VP40 and VP40 mutants. At 24 hours after transfection, cells were stained with an anti-cMyc antibody (red) and an anti-CD63 (a multivesicular body marker) antibody (green). Nuclei were stained with Hoechst (Invitrogen) (blue). L domain motif mutants were transported to the plasma membrane and colocalized with CD63 and with wild-type VP40.
