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Oligomerization of VP40 mutants. We cotransfected 293T cells with plasmids encoding the FLAG-tagged VP40 and a cMyc-tagged VP40 mutant or a FLAG-tagged VP40 mutant and the cMyc-tagged VP40. At 48 hours after transfection, the immunoprecipitation assay (A) and virus-like particle (VLP) budding assay (B) were performed. The 3940A-VP40 had no effect on oligomer formation or VLP budding induced by the wild type.
