Figure 1.

hnRNP C1/C2 is a karyopherin α1 (KPNA1) interacting protein that may be displaced by VP24. A, Coprecipitation of host cell proteins with Flag-tagged KPNA1, 2, and 3. The bands corresponding to the KPNAs and GFP are labeled. The single asterisks beside the 2 bands in figure 1A indicate bands that corresponded to hnRNPC by Mass Spectrometry analysis. B, Identification of a protein band that interacts with KPNA1 in the absence but not the presence of VP24 (band labeled with a single asterisk). The band directly above this band (labeled with 2 asterisks) is unaffected by the presence of VP24 (top). Western blot of the same lysates as above showing the interaction of VP24 interacting with KPNA1 (bottom). C, Mass spectrometry results for bands labeled with single asterisks were identified as either hnRNP C1 or C2. The complete sequence of hnRNPC1 is shown with the underlined bold text indicating the peptides that were identified by mass spectroscopy. D, FLAG-KPNA1 co-immunoprecipitates hnRNPC1/C2. Marburg virus nucleoprotein (MARV NP) was used as a negative control in this study.