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. 2011 Nov 1;204(Suppl 3):S919–S926. doi: 10.1093/infdis/jir324

Figure 2.

Figure 2.

Colocalization of Sec61α and Ebola virus proteins. Because Sec61α localizes to the ER and ER-golgi intermediate, colocalization of Sec61α and protein disulfide isomerase (PDI, an ER-specific protein) was evaluated by using an anti-Sec61α antibody (green) and an anti-PDI antibody (red; A). Colocalization of endogenous Sec61α and VP24 was tested by using an anti-Sec61α antibody (green) and an anti-VP24 antibody (red; B). In the presence of eGFP-Sec61α (green), coexpressed VP24 was detected with an anti-VP24 antibody (red; C). 293 cells coexpressed with eGFP-Sec61α and FLAG-L, NP, VP30, or VP35 were detected with an anti-FLAG antibody (D, top panel), an anti-NP antibody (D, second panel), an anti-VP30 antibody (D, third panel), or an anti-VP35 antibody (D, bottom panel), respectively. Nuclei were stained with Hoechst 33342.