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Electron microscopic analysis of nucleocapsid formation. 293 cells were transfected with siRNA against the nontarget or Sec61α. Forty-eight hours later, the cells were transfected with plasmids for the expression of NP (1 μg), VP35 (0.5 μg), and VP24 (0.5 μg). Ultrathin sections were prepared and observed using electron microscopy.
