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. Author manuscript; available in PMC: 2012 Oct 7.
Published in final edited form as: Mol Cell. 2011 Oct 7;44(1):62–71. doi: 10.1016/j.molcel.2011.06.021

Figure 3. HBO1 binding at origins is controlled by JNK1 kinase activit.

Figure 3

(A) HBO1 association at the indicated origins in cells treated with sorbitol following a pre-treatment with JNK (2 different compounds), p38 or ATM kinase specific inhibitor (see Supplemental Figure S5 for level of kinase inhibition).

(B) HBO1 association at the indicated origins during the recovery phase following an hyper-osmotic shock (right panel). JNK activity (assayed by phosphorylation) in control, hyper-osmotic and recovery stages is illustrated in the left panel.

(C) HBO1 association at MCM4 origin in cells treated with siRNAs specific for JNK1 or JNK2 prior to hyper-osmotic shock (right panel). Western blot analysis of siRNA specificity and efficiency is shown in the left panel.

(D) HBO1 association at MCM4 origin in cells treated with a JNK1-specific siRNA, prior to exposure to DTT, anisomycin, urea, hydroxyurea, actinomycin D (ActD), cisplatin, or no treatment (asynchronously growing cells).