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. Author manuscript; available in PMC: 2012 Oct 13.
Published in final edited form as: Vaccine. 2011 Aug 10;29(44):7696–7703. doi: 10.1016/j.vaccine.2011.07.133

Figure 2. EB peptide induces influenza uptake by macrophages.

Figure 2

FITC-labeled PR/8 virus (512 HAU) was mock (0 µM) or treated with increasing concentrations of EB peptide for 1 hr at 37°C. Samples were added to 2×105 RAW264.7 macrophages for 1 hr at 37°C and analyzed for virus uptake by flow cytometry. A) Histograms are presented as FITC signal of cells alone (shaded peak) or cells associated with FITC-PR/8 virus (non-shaded peak, arrows). Cells were gated by forward and side scatter. B) Median fluorescent intensity of each sample is indicated. Triplicate measurements of at least 5000 events per experimental sample and are presented as the mean ± standard deviation, and results are indicative of two independent experiments. * (p < 0.05)