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. 2001 Apr 3;98(8):4722–4727. doi: 10.1073/pnas.081062398

Figure 1.

Figure 1

Immunolocalization of F-spondin protein in E13 rat embryo. Sections were labeled with the anti-reespo domain Ab R5 (15) [tetramethylrhodamine B isothiocyanate (TRITC) in A and FITC in B]. (A) Cross section at the spinal cord level. F-spondin protein is localized in the basal membrane that underlies the floor plate (fp). Note that the crossing fibers of the commissural axons (arrow) are in contact with the F-spondin protein. (B) Cross section at the midbrain level. F-spondin protein is localized at the extracellular matrix that underlies the brain, as well as in the neuroepithelium lateral to the floor plate. (Scale bar = 25 μm; A is at the same magnification.)