Figure 5.

Knockdown of hif2α reduces the expression of the survivin orthologues. (a–c) Lateral views of hif2α (a), birc5a (b) and birc5b (c) transcription in 24 h.p.f. WT embryos. Expression of all three transcripts overlapped in the CNS region. (d–g) Lateral views of birc5a (d and f) and birc5b (e and g) expression in hif2α ATG-MO (d and e) and hif1α, hif3α dual ATG-MO (f and g) 24 h.p.f. embryos. The expression levels of both birc5a and birc5b were reduced in hif2α morphants. (h and i) Quantitative RT-PCR of the birc5a (h) and birc5b (i) genes in WT, hif2α ATG-MO and hif1α, hif3α dual ATG-MO 24-h.p.f. stage embryos. Expression was normalized to β-actin. Knockdown of hif2α caused a reduction in the transcription of birc5a and birc5b