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. 2011 May 6;18(11):1771–1779. doi: 10.1038/cdd.2011.48

Figure 6.

Figure 6

Pharmacological depletion of EZH2 phenocopies EZH2 knockdown in modulating DNA damage response. (a) FACS analysis of U2OS cells treated with ETO, DZNep or both. Cells were treated with DZNep (5 μM) for 24 h, followed by ETO (10 μM) for 48 h. (b) Western blot analysis of indicted proteins in cells treated as a. (c) ChIP analysis of EZH2 and H3K27me3 enrichment at FBXO32 promoter in DZNep-treated or -untreated cells. (d) FACS analysis of Saos-2 cells treated with ETO, DZNep or both. Cells were treated with DZNep (5 μM) for 24 h, followed by ETO (10 μM) for 48 h. (e) Western blot analysis of indicted proteins in cells treated as c. (f) Western blot analysis of indicated proteins in HCT116 cells treated with DZNep or ADR or both in the presence of MG132. (g) Western blot analysis of indicated proteins in HCT116 cells expressing a FBXO32 shRNA in response to ADR, DZNep or both. (h) Apoptosis analysis of cells treated in g. (i) A proposed model for a role of EZH2 in modulating DNA damage response