Figure 1.

Spontaneous apoptosis of differentiated Hoxb8 neutrophils of various genotypes. (a and b) Analysis of spontaneous apoptosis of Hoxb8 neutrophils by staining with AnnexinV–FITC and propidium iodide (PI) (a) or staining for active caspase-3 (b). Mature neutrophils of the indicated genotypes (differentiated for 4 days by estrogen withdrawal) were incubated in the absence of SCF for the indicated time periods and analyzed by flow cytometry. Shown are all AnnexinV-positive cells, including cells staining double positive for AnnexinV and PI (a). Data are mean/S.E.M. of three experiments. (c) AnnexinV/PI staining of differentiated wt neutrophils SCF deprived for 24 h in the presence of the pan-caspase inhibitor qVD-OPH (50 μM) or DMSO as vehicle control (veh). Data are mean/S.E.M. (n=3 experiments)