Table 1.
Characteristics of Cell Lines1
| Cell Line | Cell Doubling Time (h) | %S Phase Cells | % Ki67-Labeled Cells | % BrdU-Labeled Cells | TK1 Activity (fmol dTMP/30’/ug protein) | hENT1 Sites (107 sites/ug protein) |
|---|---|---|---|---|---|---|
| TK6 (parent) | 20.4 ± 0.7 | 45.9 ± 2.3 | 90.7 ± 2.1 | 46.9 ± 2.9 | 193.9 ± 9.7 | 7.0 ± 1.5 |
| Control Clones | ||||||
| TK6-Clone T02 | 19.1 ± 0.3 | 55.1 ± 3.6 | 70.4 ± 5.1 | 46.4 ± 2.4 | 298.1 ± 46.1 | 5.7 ± 0.1 |
| TK6-Clone T03 | 19.0 ± 0.3 | 57.1 ± 2.8 | 75.0 ± 7.0 | 46.0 ± 2.3 | 345.6 ± 17.1 | 6.8 ± 0.6 |
| TK6-Clone T04 | 20.3 ± 0.5 | 57.7 ± 2.9 | 73.1 ± 6.3 | 39.2 ± 0.5 | 379.9 ± 34.8 | 5.3 ± 0.1 |
| TK6-Clone T05 | 19.2 ± 0.4 | 59.7 ± 2.3 | 73.1 ± 2.8 | 43.4 ± 1.2 | 289.3 ± 23.6 | 3.9 ± 0.2 |
| Mean ± sem | 19.4 ± 0.3 | 57.4 ± 1.0 | 72.9 ± 1.0 | 43.8 ± 1.7 | 328.2 ± 21.2 | 5.43 ± 0.6 |
| Trifluorothymidine-Resistant Clones | ||||||
| TK6-Clone T2 | 21.3 ± 0.7 | 42.8 ± 4.0 | 84.8 ± 2.5 | 0.5 ± 4.0 | 5.0 ± 1.8 | 3.8 ± 0.1 |
| TK6-Clone T4 | 18.3 ± 0.0 | 47.9 ± 1.8 | 87.3 ± 0.0 | 0.8 ± 1.8 | NMA | 3.3 ± 0.2 |
| TK6-Clone T13 | 21.8 ± 0.2 | 43.6 ± 1.7 | 62.8 ± 10.1 | 2.4 ± 0.8 | NMA | 8.9 ± 0.3 |
| TK6-Clone T14 | 20.6 ± 0.1 | 42.5 ± 1.7 | 58.4 ± 6.5 | 2.4 ± 0.9 | NMA | 11.5 ± 0.3 |
| Mean ± sem | 20.5 ± 0.8 | 44.2 ± 1.3 | 73.3 ± 7.4 | 1.5 ± 0.5 | 1.3 ± 1.3 | 6.9 ± 2.0 |
1
TK6 is the parent cell line, TK6-T2 and TK6-T4 are independent TRIFLUOROTHYMIDINE-resistant clones, and the remaining cells are independently isolated TK6 clones.
2
fmol dTMP/30’/μg protein
3
107 sites/ug protein
4
No measureable activity