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Relation Between Protein Concentration, Fluorescence at pH 2 and at pH 7, and the pH50 Obtained
| Concentration (mg mL−1) | Fluorescence ratio pH 2/pH 7 | pH50 |
|---|---|---|
| 0.05 | 5 | 2.75 ± 0.04 |
| 0.1 | 10 | 2.67 ± 0.04 |
| 0.2 | 17 | 2.67 ± 0.03 |
| 0.4 | 29 | 2.71 ± 0.04 |
As a function of protein concentration, the ratio of ANS fluorescence intensities at pH 2 and pH 7 increases, whereas the pH50 remains within error.
