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. Author manuscript; available in PMC: 2012 Oct 5.
Published in final edited form as: Cell Metab. 2011 Oct 5;14(4):545–554. doi: 10.1016/j.cmet.2011.08.012

Figure 2. Characterization of purified Peredox.

Figure 2

(A) Green to red fluorescence ratios at the indicated pH, plotted against R′ or R (above the plot), with 80 μM NAD+ at 25°C. (B) Fluorescence ratios at the indicated pH, temperature, and ATP:ADP ratios (low, 0.3; high, 3.6; with total adenine nucleotides of 4.6 mM), plotted against R′ or R (above the plot), with 80 μM NAD+. Fluorescence ratios (mean ± SEM, n = 3) were normalized to the control condition in the absence of pyridine nucleotides at pH 7.2. (C) Kinetics of fluorescence signal upon addition of pyruvate and LDH to Peredox pre-equilibrated with saturating NADH at 25°C or 35°C, normalized to initial and final values.