Skip to main content
. Author manuscript; available in PMC: 2012 Oct 5.
Published in final edited form as: Cell Metab. 2011 Oct 5;14(4):545–554. doi: 10.1016/j.cmet.2011.08.012

Figure 4. Cultured mouse neuroblastoma Neuro-2a cells supplied with glucose show a more reduced cytosolic NADH-NAD+ redox state.

Figure 4

(A) Steady state fluorescence responses of Neuro-2a cells plotted against concentrations of extracellular glucose (mean ± SEM, n = 19 cells from two independent experiments). (B) Steady state fluorescence responses of Neuro-2a cells plotted against total concentrations of extracellular lactate and pyruvate, with a constant lactate:pyruvate ratio of 10, and glucose of 10 mM or 0 mM (mean ± SEM, n = 7 cells from three independent experiments). For the alternate y axis, the predicted NAD+:NADH ratio was calculated from purified protein measurements. p < 0.001 (paired t-test) for all conditions in 10 mM vs. 0 mM glucose.