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. Author manuscript; available in PMC: 2012 Oct 7.
Published in final edited form as: Mol Cell. 2011 Oct 7;44(1):134–146. doi: 10.1016/j.molcel.2011.06.038

Figure 1. p62 interacts with Raptor and components of the mTORC1 complex, but not of mTORC2.

Figure 1

(A) LC/MS/MS spectra of the purified Flag-p62 peptides corresponding to raptor from NIH-3T3 cells stably expressing Flag-p62. (B) Lysates and Flag immunoprecipitates from NIH-3T3 and 293 cells stably expressing Flag-p62 were analyzed to detect the indicated proteins. In (C) through (G) 293 cells were transfected with the indicated cDNAs in expression vectors, cell lysates were prepared, and lysates and HA-, Flag-, or myc-tagged immunoprecipitates were analyzed by immunoblotting to quantify the specified ectopic proteins. (H) Endogenous interaction of p62 with components of the mTOR signaling pathway in response to amino acids or leucine. Cell lysates and p62 immunoprecipitates from amino acid- or leucine-treated HEK293T cells were analyzed for the levels of specified proteins. These results are representative of three experiments.