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. Author manuscript; available in PMC: 2012 Oct 7.
Published in final edited form as: Mol Cell. 2011 Oct 7;44(1):134–146. doi: 10.1016/j.molcel.2011.06.038

Figure 2. p62 is essential for mTORC1 activation in response to amino acids.

Figure 2

Effects of p62 depletion on activation of the mTORC1 pathway in response to (A) amino acids or (B) insulin. p62fl/fl MEFs were infected with GFP or CRE adenoviruses, and then stimulated with amino acids or insulin. Lysates were analyzed for levels of the specified proteins. (C) WT and p62KO MEFs, reconstituted with p62FL or p62CT, were treated as described in (A). Cell lysates were prepared and analyzed by immunoblotting for the levels of the indicated proteins. (D) 293 cells stably expressing Flag-p62 were deprived of amino acids and serum for 50 min, pretreated with rapamycin for 30 min, and then stimulated with amino acids for the indicated durations. Cell lysates were analyzed for the levels of the specified proteins. These results are representative of three experiments.