Figure 1. M290-SAP efficiently depletes CD103+ cells in vivo.
Normal C57BL/6 mice were untreated or injected i.p. with the indicated reagents at 2mg/kg i.p. Six days later, cells from the indicated compartments were isolated and subjected to multi-color FACS analyses using mAbs to CD3, CD4, CD8, CD11c, and CD103. A. Percentages of residual CD103+CD8+ cells in different compartments. Numbers in each quadrant represent percentages of cells within the CD3+ T lymphocyte gate. Data are representative of at least three independent experiments. Bold numbers at middle right of each quadrant are the frequencies of CD103-expressing cells as a percentage of total CD8 T cells. B. Absolute numbers of CD4 and CD8 T cells in MLN from A. Data shown are mean ± SEM from at least four independent experiments. Statistically significant differences from the PBS-treated mice were measured by one-way ANOVA with posttesting by Dunnett’s Multiple Comparison Test. ***denotes p-values<0.001, * denotes p-values<0.05.