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. 2001 Mar 27;98(8):4734–4739. doi: 10.1073/pnas.081553598

Figure 4.

Figure 4

Aβ1–42 blockade of chimeric α7-nAChR/5HT3 receptors. HEK293 cells were transfected with either the chimeric α7-nAChR/5HT3 receptor (A) or wild-type 5HT3 receptor (B) constructs and were examined 2 days later with whole-cell patch–clamp recording to compare responses elicited by ACh or 5-HT in the absence (Left) and presence (Right) of 100 nM Aβ1–42 for cells voltage-clamped at −60 mV. The black bar of the construct represents the α7 domain; the gray bar represents 5HT3 wild-type domain; M indicates transmembrane domain. The peptide produced significant (and equivalent) inhibition of chimeric responses elicited by either 0.3 or 1 mM ACh but not wild-type responses elicited by 1 mM 5HT (C). The peak amplitude of the response in Aβ1–42 was normalized to the initial response from the same cell in each case (n = 10 for each; P < 0.001).