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PU.1 siRNA treatment suppresses PU.1 protein levels and induces hemoglobin expression. GATA-1-ER MEL cells were treated with 10−7 mol/L of β-estradiol or transiently transfected with a double-stranded PU.1 siRNA or control siRNA at the concentrations indicated for 26 h. Cell extracts were prepared by sonication, separated by SDS-PAGE, and immunoblotted for PU.1 (20 µg/lane; A) or hemoglobin (9 µg/lane; B). C. Quantitation of PU.1 and hemoglobin immunoblot band intensities.
