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. Author manuscript; available in PMC: 2011 Oct 11.
Published in final edited form as: Mol Cancer Res. 2007 Oct;5(10):1053–1062. doi: 10.1158/1541-7786.MCR-07-0145

FIGURE 3.

FIGURE 3

PU.1 siRNA treatment induces a transient suppression of PU.1 protein levels. A. GATA-1-ER MEL cells were transfected once with 80 nmol/L of PU.1 siRNA. Cell extracts were prepared by sonication at the indicated times, separated by SDS-PAGE (20 µg/lane), and immunoblotted for PU.1. B. GATA-1-ER MEL cells were repeatedly transfected with PU.1 siRNA or control siRNA as described in (A) every 18 h. Extracts of the cells were prepared by sonication at the indicated times, separated by SDS-PAGE and immunoblotted for PU.1.