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. Author manuscript; available in PMC: 2011 Oct 11.
Published in final edited form as: Mol Cancer Res. 2007 Oct;5(10):1053–1062. doi: 10.1158/1541-7786.MCR-07-0145

FIGURE 6.

FIGURE 6

Inhibition of PU.1 synthesis leads to terminal cell division. GATA-1-ER MEL cells were repeatedly transfected with 80 nmol/L of PU.1 or control siRNA every 18 h. At 24-h intervals they were plated as single cells in plasma clots in the absence of siRNA and incubated for an additional 4 days at 37°C as described in Materials and Methods. The clots were then transferred to glass slides and stained for hemoglobin expression with benzidine reagent and counterstained with hematoxylin. Small benzidine-positive colonies represent committed cells undergoing terminal cell division whereas large benzidine-negative colonies represent uncommitted cells. Representative colonies in plasma clots from cells treated for 3 days with the indicated siRNAs. Arrows, uncommitted colonies; arrowheads, colonies committed to differentiation.