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. 2011 Sep 29;10:171. doi: 10.1186/1476-511X-10-171

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Copyright ©2011 Basu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Semi-quantitative RT-PCR analysis of WT1 splice variants (KTS+/KTS-) in human kidney and non-kidney sourced adipose stromal cells. All lanes were normalized by total mass of cDNA: molecular weight ladder used for sizing (1), lipoaspirate stromal cells (Lane 2), subcutaneous adipose stromal cells (Lane 3), major calyx adipose stromal cells (Lane 4), renal pedicle stromal cells (Lane 5). Expression of WT1 is only detectable from renal adipose. Note that the ratio of KTS+/KTS- splice variants differs between major calyx and renal pedicle derived cell sources derived from the same donor.