Figure 9.

Expression of EPO from human kidney and non-kidney sourced adipose stromal cells is comparable with primary renal cells, hepatocytes and keratinocytes. Kidney and non-kidney sourced adipose stromal cells express distinct isoforms of EPO distinguishable though isoelectric focusing gel electrophoresis and western blotting. Samples are as follows: human keratinocytes (1), hepatocytes (2), renal- (3) non-renal- (4) adipose stromal- cells and primary renal-cells all under normoxia (5), primary renal cells under hypoxia (6) and HepG2 cells as a positive control (7). Blot was probed with anti-EPO monoclonal antibody for Western analysis. Comparison of lanes 5 and 6 shows clear up-regulation of EPO protein expression in response to hypoxia from primary renal cell isolates. All lanes were normalized by total mass of protein (10 μg).