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. 2011 Aug;29(4):415–419. doi: 10.3329/jhpn.v29i4.8459

Table 1.

Validation and repeatability of measurement using Hipp baby formula by HPLC

Sample name Ret time min Area Mv min Height Mv Amount ppb % of recovery
Std 2.0 ppb 5.48 221,323 878,118 2.00001 100
Std 1.0 ppb 5.49 110,008 43,988 1.0003 100
Std 0.5 ppb 5.47 54,896 219,905 0.4985 99.7
Std 0.25 ppb 5.48 27,419 111,156 0.2504 100.16
Spiked milk
2.5 ppb (1) 5.57 55,298 246,355 2.23 89
             (2) 5.57 56,604 256,889 2.33 93
             (3) 5.57 59,533 27,180 2.58 103

HPLC=High-performance liquid chromatography;

min=Minutes;

Mv=Measurement unit;

Ret=Retention;

Std=Standard; 1, 2, and 3 with 2.5 ppb indicate replicate 1, 2, and 3