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. 2011 Nov 1;138(21):4801–4812. doi: 10.1242/dev.070912

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Fig. 6. — Genetic fate-mapping studies indicate that ER71-expressing cells give rise to the endothelial and hematopoietic lineages. (A) The transgenic Er71-Cre mouse model used for these studies. (B,C) Immunohistochemistry for GFP (green) and desmin (red) and DAPI staining (blue) in E11.5 (B) and P3 (C) heart. a, atria; bw, body wall; cu, cardiac cushion; fl, fetal liver; v, ventricle. (D) A representative FACS profile showing EYFP⁺ versus side scatter width (SSW). (E) Percentage of Rosa-EYFP⁺ cells from all experiments (n=9). (F-H) EYFP-positive cells were gated and analyzed for lineage contribution. Representative FACS profiles are shown for (F) endothelial lineage (CD41^– CD45^– Tie2⁺) cells and (G) CD41⁺ CD45⁺, (H) CD45⁺ Ter119^– and Ter119⁺ CD45^– hematopoietic lineage cells. (I) Percentage of Rosa-EYFP⁺ cells of each lineage from all experiments (n=9). Error bars indicate s.e.m.