Fig. 8.

Overexpression of ER71 in differentiating EBs inhibits cardiac development. (A-D) Sections of representative EBs either untreated (A,C) or induced to overexpress ER71 (B,D) stained with Hematoxylin and Eosin (C,D) or anti-TnT (A,B). (E) Quantitative analysis of the troponin T-positive area in 33 induced (Dox) and 42 untreated sections. (F) qRT-PCR confirms that representative cardiac program transcripts (Nkx2-5, Tbx5, Gata4) are downregulated in ER71-overexpressing EBs. (G,H) Extent of contraction and relaxation after 2 seconds of stimulation (G) and percent shortening (H) are reduced in ER71-overexpressing EBs. ^****, P<0.001; ^*, P<0.05. Error bars indicate s.e.m.