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. 2001 Apr 3;98(8):4740–4745. doi: 10.1073/pnas.081536298

Figure 3.

Figure 3

Strategy for targeted disruption of the DGKɛ gene in the R1 ES cell line and C57/BL6 mice. (A) Parental and targeted DNA fragments after digestion with XbaI in ES cell lines. (B) Southern blot analysis of selected ES cell lines. Untargeted cell lines were 2f7 and 2f8. Targeted cell lines were 2c7, 2d2, 2e5, 2e9, and 2f9. (C) Southern blot analysis of wild-type and heterozygous mutants by using tail DNA extracts. The 15-kb band is wild type, and the 10.5-kb band is the targeted deletion of DGKɛ.