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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: J Immunol. 2011 Sep 14;187(8):4178–4186. doi: 10.4049/jimmunol.1101201

Figure 3. IRBP1-20 immunization induces a greater IFN-γsecreting Th1 subset in the dko mice than in the wild-type.

Figure 3

Pooled spleen and draining lymph node of CD4+ T cells and splenic APCs were isolated from naïve (0pi) mice and from mice at day 14pi or day 21pi with 250 μg of IRBP1-20 peptide in CFA per mouse, then 4×105 CD4+ T cells and syngeneic 1×105 APCs were co-cultured for 48 h in the presence of IRBP1-20 and levels of IFN-γ (A) and IL-17A (C) in the culture medium were measured by Ready-setgo ELISA kits (eBiosciences). B, qPCR analysis of IFN-γ mRNA in total RNA prepared from the spleen (top) or lymph nodes (bottom) of IRBP1-20-immunized WT or dko mutant mice at day 0pi, 14pi, and 21pi. The results are the mean ±SD for 5 wells (each containing pooled cells from 3 mice) per group in A and C and for 3 mice per group in (B) in a single experiment and are representative of those in three independent experiments. **p<0.001 by the one-way ANOVA test using ProStat Ver 5.5.