Figure 3.

The rostral migratory stream in the adult macaque monkey olfactory peduncle (OP). (a) A sagittal plane reveals that BrdU-labeled nuclei (arrows, green) are aligned in a strict linear pattern within the core of the peduncle. GFAP immunoreactivity (blue) in the OP is especially pronounced along this pathway. These BrdU-labeled nuclei are immunonegative for the mature neuronal marker, NeuN (red), unlike the neurons seen in the orbitofrontal cortex (OFC) and the few scattered neurons at the periphery of the peduncle that belong to the anterior olfactory nucleus. (b–d) Images in same x, y, and z registration show chains of neuroblasts in the rostral migratory stream. Within the peduncle, chains of intensely stained TuJ1-positive cells (red) are restricted to the core and are aligned parallel to the longitudinal axis. (c) Some cells within these chains have elongated BrdU-positive nuclei (arrows, green) oriented in the presumed axis of migration. (d) These chains are immediately surrounded by a sheath of GFAP-positive fibers (blue) but are GFAP-negative. (e–g) The TuJ1-positive chains in the peduncle (e, red) also coexpress PSA-NCAM (f, green) as indicated by yellow fluorescence when the two signals are merged (g). [Bars = 100 μm (a) and 25 μm (b–g).]